DNA Transformation of Bacteria: Ampicillin Resistance
Genes control the traits that living organisms possess. Bacteria, such as E. coli, have genes on their chromosome and on a small circular piece of DNA called a plasmid. Genes can be transferred from one bacteria to another on the plasmid by a process known as transformation.
In this experiment, a plasmid with a gene (DNA) for resistance to the antibiotic ampicillin will be used to transfer the resistance gene into a susceptible strain of the bacteria. The same technique is used to transfer genes (DNA) for the production of insulin, growth hormones, and other proteins into bacteria.
3 class periods, including incubation time